Invitrogen lr clonase manual

Description. Gateway LR Clonase II enzyme mix catalyzes in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and destination vector (containing att R sites) to generate an expression clone. Gateway LR Clonase II contains enzymes and buffer in single mix to enable convenient 10mL reaction set up with fewer pipetting www.doorway.ru: InvitrogenTrade. Thaw on ice the LR Clonase™ II enzyme mix for about 2 minutes. Vortex the LR Clonase™ II enzyme mix briefly twice (2 seconds each time). 3. To each sample (step 1), add 2 μL of LR Clonase™ II enzyme mix to the reaction and mix well by vortexing briefly twice. Microcentrifuge briefly. 4. Return LR Clonase™ II enzyme mix to –20°C or –80°C www.doorway.ru Size: KB.  · The recombination (att) sites of each vector comprise a hybrid sequence, donated by the sites on the parental vectors. The recombination can occur between DNAs of any topology (supercoiled, linear, or relaxed), although efficiency varies. The GATEWAY LR Cloning Reaction The LR reaction is used to create an Expression Clone (Figure 3).

The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes ) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months. For long-term storage, store at −85°C to −68°C. Thaw on ice the Invitrogen LR Clonase II enzyme mix for about 2 minutes. Vortex the LR Clonase II enzyme mix briefly twice (2 seconds each time). To each sample (Step 1, above), add 2 µl of LR Clonase II enzyme mix to the reaction and mix well by vortexing briefly twice. Microcentrifuge briefly. the Gateway® Technology with Clonase™ II manual. This manual is available for downloading from our Web site (www.doorway.ru) or by contacting Technical Support (page 20). attP Sequence Variations The attP sites between the pDONR™ vectors will contain slight sequence variations which do not affect the specificity of recombination.

Description. Gateway LR Clonase II enzyme mix catalyzes in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and destination vector (containing att R sites) to generate an expression clone. Gateway LR Clonase II contains enzymes and buffer in single mix to enable convenient 10mL reaction set up with fewer pipetting steps. The Donor Vector box, the Gateway® BP Clonase® II Enzyme Mix and Reagents box, and the M13 Sequencing Primers box (Boxes 1–4) contain the following items. Store the Gateway® BP Clonase® II enzyme mix at −30°C to −10°C for up to 6 months. Thaw on ice the LR Clonase™ II enzyme mix for about 2 minutes. Vortex the LR Clonase™ II enzyme mix briefly twice (2 seconds each time). 3. To each sample (step 1), add 2 μL of LR Clonase™ II enzyme mix to the reaction and mix well by vortexing briefly twice. Microcentrifuge briefly. 4. Return LR Clonase™ II enzyme mix to –20°C or –80°C storage.